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Enzyme The plot of enzyme activity Vs. pH is often "bell shaped" since two different amino acid groups of the enzyme are being titrated to different states of ionization at the different pH values. This means that only one of the two possible ionization states of the amino acid side chain is effective in enzyme catalysis - in other words, if an acidic side chain like Glu is involved in catalysis, it probably only works when it is ionized; since Glu side chain carboxylic acid groups have a pK around pH 5, at pH values below this pK, the enzyme will be less active; while at pH values above the pK, the enzyme Approximate words: 1731 Approximate pages: 7

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